Herbicide Resistant Transgenic Wheat Plants Obtained by Microprojectile Bombardment
The efficient short procedure was employed for obtaining wheat (Triticum aestivum) transgenic plants from appropriate commercial cv (Oasis). First, optimum conditions were developed for the generation of embryogenic calli from immature embryos that have a high regeneration level. High speed microprojectile bombardment was used for transforming calli, using the plasmid pAHC 25 which contains the reporter gene ?-glucuronidase (GUS) and the selectable BAR gene, which resists Basta herbicide. The transformations have been verified by ?-glucuronidase, PCR and Southern blot testing. It was highly efficient and comparable to other reports used in the "crop" cultivar. The complex genetic modification of wheat (Triticum aestivum) and other floral organisms with a relatively low efficacy of biolistic processing and the resulting introduction of multiple copies of the genes are being added. Southern work confirmed further integration of gus and bar genes into the genomes of durum wheat. Developed by an agronomically superior durum cultivar this transformation technique will provide new opportunities for enhancing the current germplasm through biotechnology.