RAPID MULTIPLICATION OF ORNAMENTAL BULBOUS PLANTS OF Lilium orientalis AND Lilium longiflorum

Abstract

Lilium is one of the most important cut flowers all over the world. It ranks within the ten top most beautiful cut flowers because of its attractive and large showy flowers. Ornamental lily hybrids have been developed in very large amount. Effect of different growth hormones and different growth media was observed in this study. Bulb scales were used as an explant, cultured in vitro on Murashige and Skoog (MS) medium containing different concentrations of 6-benzyladenine (BA) and naphthalene acetic acid (NAA). Among different treatments used for plant culturing, the MS medium supplemented with 6-benzylaminopurine (BAP) 3.0 mg/L was found to be the best for shoot initiation from scales of bulbs. After that plants were transferred to different media for multiple shooting. Out of different concentrations used the medium with 1.0 mg/L BAP and 1.0 mg/L NAA increased frequency of shoot formation up to 90%. An average of about 10 3.73 roots/explants with well-developed roots and bulblet formation were obtained in this medium.