Chemical Investigation of Medicinal Plant Allium Sativum L. Family (Amaryllidaceae,) from Sindh Pakistan

Abstract

The most famous medicinal plants were selected for study; Allium sativum L. family  (Amaryllidaceae,) Local name in Urdu  (Lahsan)  English name (Garlic)  Habit Bulb  Parts used Whole plants The tap water was used for washing the plant material (seeds).  Dry under shade for one week at least and grinded by means of grinding machine. Put this powdered form of plant samples in vial. Manage all these chemical tests on plants samples (which are now in powder form) by using SEM/EDS laboratory. The elemental determination of samples takes place in pure and applied Geology, University of Sindh Jamshoro. The elemental analysis determination of the samples. In result we see the elements which are present in Allium sativum L are. potassium, calcium, phosphorus, sulfur, magnesium, silicon, aluminum. The material (seeds) were carried in laboratory for fatty acid analysis. . Sulfur 1.48 Magnesium 1.02 Silicon 0.15 , Potassium 0.34 Phosphorus  0.08 , Calcium 0.06, Aluminum , 0.05 .These plants (seeds) were soaked in  chloroform + n-hexane (1:1).By rotary evaporator the extract of plants were evaporated for column chromatography and FAME, then the sample were carried for GC-MS isolation of fatty acids from  Alium sativum L. total  11 compounds were isolated  4 = Saturated, 1  Unsaturated, 6 Hydrocarbon compound. Octadeadienoate (Unsaturated  fatty acid) present in the  highest amount 39.5% Palmitate (saturated  fatty acid ) 19.1%  Nonadecanoate  (Saturated  fatty acids ) 15.1% , Behenate (saturated  fatty acid) 3.55%  Arachidate  (saturated  fatty acids ) 2.77% and six different compounds were isolated.